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G418 (Geneticin, hereditary mycin) is an aminoglycoside antibiotic. It is the most commonly used resistance screening reagent for stable transfection in molecular genetic test. It blocks protein synthesis by inhibiting transposon Tn601 and Tn5 genes, interfering with ribosome function, and produces toxins to prokaryotic and eukaryotic cells, including bacteria, yeast, plant and mammalian cells, as well as protozoa and worms. When neo gene is integrated into eukaryotic DNA, it can activate the coding sequence of neo gene to transcribe into RNA, thus obtaining the high expression of aminoglycoside phosphotransferase, which is the resistant product, so that cells can acquire resistance and grow in selective medium containing G418. This selective characteristic of G418 has been widely used in gene transfer, gene knockout, resistance screening and transgenic animals.